Comparative immuno-reactivity of recombinant non-structural protein 2 fragments (N- and C- terminus) to detect bluetongue viral antibodies in small ruminant serum samples

Nirmal Chacko,Sanchay Kumar Biswas,Nihar Nalini Mohanty,Karam Chand,Awadh Bihari Pandey,Bimalendu Mondal,Sathish Bhadravati Shivachandra

Small Ruminant Research（2019）

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摘要

•Production of recombinant non-structural protein 2 fragments of BTV; rNS2Nt and rNS2Ct.•Evaluation of diagnostic efficacies of rNS2Nt and rNS2Ct fusion proteins in ELISA format.•Detection of bluetongue viral NS2 specific antibodies in ruminant serum samples.•rNS2 N-terminus protein indicated better immuno-reactivity than rNS2 C-terminus protein.

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关键词

Bluetongue virus,Recombinant non-structural protein 2,N- and C- terminus,Indirect-ELISA,Antibodies,Small ruminant serum

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