Egg proteins stabilized green silver nanoparticles as delivery system for hesperidin enhanced bactericidal potential against resistant S. aureus

Nano-carriers enhance drugs stability and solubility and prevent their rapid degradation. Silver nanoparticles (AgNPs) are preferable for drug delivery applications due to their unique properties and less toxicity. Protein AgNPs are versatile nano-carriers as they provide the anchoring layer for drug molecules to be transported into cells. Herein, the development of a simple method for the synthesis of egg proteins stabilized AgNPs and their use as nano-carrier for hesperidin is reported. Protein stabilized AgNPs were characterized through UV–Vis spectrophotometer, FT-IR, zetasizer and atomic force microscope (AFM). The bactericidal potentials of hesperidin loaded AgNPs were studied against sensitive and multidrug-resistance bacteria strains via Tetrazolium Microplate Assay. UV–visible spectrum showed peak that corresponds to the plasmon absorbance of AgNPs. FT-IR study reveals bands which indicate effective capping of AgNPs with egg proteins. AgNPs were found spherical in nano-range size with negative charge and loaded increased amount of hesperidin. An increased bactericidal activity of hesperidin was observed against tested bacteria strains upon loading in AgNPs. Current findings suggest a facile approach for proteins stabilized AgNPs and their potential as effective nano-carrier for enhancing the bactericidal activity of hesperidin.