Low CD3+and CD73+Lymphocyte Counts, and High Neutrophil/ CD73 Ratio During TTM Are Associated With Poor Outcome After Cardiac Arrest

Introduction: Immune cell activity modulates inflammation and may affect outcomes in post-resuscitation syndrome. We characterized patterns of immune cells circulating in peripheral blood at different time points to establish immune “phenotypes” of patients with good and poor outcomes, and to investigate immune mechanisms of disease after cardiac arrest. Hypothesis: Circulating lymphocyte subpopulations may be associated with outcome after cardiac arrest. Methods: After informed consent, blood samples were collected at 6, 12, 24, 48, 72, and 168 hours after resuscitation. White blood cells were isolated, counted and analyzed for the expression of specific pro-inflammatory antigens using flow cytometry. Cell counts at each time point were correlated with neurological outcomes determined at 3-12 months, and outcomes were classified as good (CPC 1-2) or poor (CPC 3-5) by convention. Results: The 34 research subjects were 55.9±16.6 years old and 23.5% female. Median ischemic time was 17(10-22.5) minutes. Of t...