Alginatibacterium Sediminis Gen. Nov., Sp. Nov., A Novel Marine Gammaproteobacterium Isolated From Coastal Sediment

A Gram-stain-negative, strictly aerobic, rod-shaped motile bacterium with a single polar flagellum, designed strain ALS 81(T), was isolated from sediment sample collected in the AiLian Bay of Weihai, China. Phylogenetic analysis based on 16SrRNA gene sequence indicated that strain ALS 81(T) forms a lineage within the family Alteromonadaceae that is distinct from the most closely related genera Agarivorans (93.9-94.4 % 16S rRNA gene sequence similarity), Celerinatantimonas (92.0 %) and Aliagarivorans (91.6 %). Optimum growth occurred in the presence of 3-4 % (w/v) NaCl, at pH 8.0 and at 28 degrees C. The major fatty acids (>10 % of total fatty acids) were iso-C-16:0 (27.3 %), anteiso-C-15:0 (27.0 %) and anteiso-C-17:0 (11.2 %). The predominant polar lipids were phosphatidylethanolamine, glycolipid and four unidentified polar lipids. The DNA G+C content of strain ALS 81(T) calculated on the basis of the genome sequence is 43.5 mol% and the genome size is 4.5 Mbp. The predominant isoprenoid quinone is ubiquinone 8. On the basis of polyphasic analysis, strain ALS 81(T) is considered to represent a novel species in a novel genus of the family Alteromonadaceae, for which the name Alginatibacterium sediminis gen. nov., sp. nov. is proposed. The type strain of the type species is ALS 81(T) (=KCTC 62397(T) =MCCC 1K03481(T)).