One step DNA amplification of mammalian cells in picoliter microwell arrays

Wenwen Liu,Zhao Li,Yuanjie Liu,Qingquan Wei,Yong Liu,Lufeng Ren,Chenyu Wang,Yude Yu

RSC ADVANCES（2019）

引用 8|浏览46

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摘要

We developed a strategy for direct DNA amplification of single cells on a PEG-modified silica chip with 30 600 picoliter-sized microwells. HPV-positive cells in heterogeneous populations were successfully detected with high accuracy sensitivity as high as single copy.

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