A microfluidic-SERSplatform for isolation and immuno-phenotyping of antigen specific T-cells

Shuvashis Dey,Ramanathan Vaidyanathan,K. Kamil Reza,Jing Wang,Yuling Wang,Hendrik J. Nel,Soi-Cheng Law, Jennifer Tyler,Jamie Rossjohn,Hugh H. Reid,Abu Ali Ibn Sina,Ranjeny Thomas,Matt Trau

Sensors and Actuators B: Chemical（2019）

引用 9|浏览51

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摘要

•A microfluidic platform for the specific isolation of antigen specific T-cells with high sensitivity (0.005%).•Release of T-cell from the device using a single point direct current (DC) pulse.•Monitoring TCR expression heterogenity on antigen specific T-cells using SERS.•T-cell receptor expression analysis on individual T-cells using SERS-nanotags.

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关键词

T-cell,Microfluidics,SERS,pMHC,TCR

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