Withanolides and 26-Hydroxylated Derivatives with Anti-Inflammatory Property from Solanum Capsicoide

A phytochemical investigation of the leaves of Solanum capsicoides resulted in the isolation and characterization of five new withanolide-type steroids, capsisteroids G-K (1-5), along with three known analogues (6-8). Their structures were identified by interpretation of the NMR and HRESIMS data as well as by spectral data comparison with known analogues. The isolated steroids were found to be not cytotoxic against a limited panel of cancer cell lines. The ability of the isolated steroids to suppress superoxide anion generation and elastase release in N-fonnyl-methionyl-leucyl-phenylalanine/ cytochalasin B (fMLF/CB)-induccd human neutrophils was also evaluated and displayed variable activities.