3D Localization of Neurons in Bright-Field Histological Images

In this paper, we present a method for inferring the depth of neurons found in a bright-field microscopic image of a histological section of a human brain, digitized at high resolution in multiple planes along the z-axis. Individual neuron bodies are segmented and tracked throughout the depth of the whole image stack and placed at the appropriate z-level in the stack based on variations in image sharpness.