Exosomes Isolated From Human Inducible Pluripotent Stem Cell-Derived Cardiomyocytes Enhance Angiogenesis In-Vitro; A New Potential Therapy For The Post-Ischemic Heart

Introduction: Due to the relative poor regenerative properties of cardiac tissue, acute ischemic events can lead to progressive terminal heart failure over time unless cardiac regeneration can be affected. Recent evidence using stem cell therapy in the heart demonstrates a greater role of their paracrine signaling potential, as cell survival post-transplantation is very low. A growing body of evidence demonstrates that exosomes secreted by stem cells stimulate angiogenesis, provides cytoprotection, and modulates apoptosis. Hypothesis: The main objective of this study was to determine the angiogenic potential of exosomes isolated from human-inducible pluripotent stem cell -derived cardiomyocytes (hiPSC-CMs). Methods: hiPSC-CMs were cultured for 48 hours (21% O2, 5% CO2) with exosome-free serum (10%). At 48 hours after the culture, media was collected and exosomes were isolated using ExoQuick-TC reagent (System Biosciences kit). Isolated exosomes were characterized by Nanosight (size and concentration), wes...