Human mercaptalbumin and nuclear factor kappa B in subjects with mildly impaired renal, vascular and metabolic health compared to “super-healthy” subjects: the BIOCLAIMS Integration study

The redox state of human albumin is known to be affected by several diseases, while data is limited on activation of NF-kB, and absent in mildly impaired human health. The aim of this study was to compare 4 contrasting groups of the BIOCLAIMS cohort: mildly impaired renal health (group 1, estimated glomerular filtration rate, MDRD, 30-60 ml/min/1.73 m2, n=43), vascular health (group 2, intima-media thickness, IMT, left and right u003e75th percentile, n=59), metabolic health (group 3, HOMA index u003e2.5 and HbA1c 38.8 -44 mmol/mol, n=44), and “super” healthy subjects (group 4, all clinical chemistry variables within normal range 10%, IMT not u003e75th percentile at both sides, not on medications, n=56). Plasma human mercapt-(HMA) and non-mercaptalbumin (HNA-1) were determined by HPLC; activation of NF-kB in PBMC by TransAM Active Motif assay. ANCOVA, with age (group 1u003e2,4; 3u003e4) as covariate, showed that HMA and HMA:HNA-1 ratios were in group 1 3 (P4 (P