Thermotherapy for Na+/I− symporter-expressing cancer using anti-Na+/I− symporter antibody-conjugated magnetite nanoparticles

In this study, an anti-NIS antibody-conjugated magnetite nanoparticle (NIS-MNP), which is enhanced on T2-weighted magnetic resonance imaging (MRI) and generates heat in an alternating magnetic field (AMF), was synthesized as an alternative NIS targeting therapy. Our aim was to investigate the feasibility of using NIS-MNPs as a diagnostic and therapeutic agent for DTC by studying NIS-MNP in an NIS overexpressing HeLa (HeLa-NIS) cell line and a subcutaneous HeLa-NIS tumor-bearing mouse model. Results showed that NIS-MNP bound effectively to the surface of HeLa-NIS cells, and AMF inhibited cellular growth of NIS-MNP-treated HeLa-NIS cells. Normal thyroid glands of mice were enhanced by NIS-MNP treatment in T2-weighted MRI. Inhibition of tumor growth was observed after AMF irradiation in the NIS-MNP group.