Analyses of extracellular protein production in Bacillus subtilis – II: Responses of reaction network to oxygen transfer at transcriptional level

•iBsu1144rhGH was reconstructed based on recent resequencing of B. subtilis genome.•Effects of oxygen transfer on transcriptome of B. subtilis were elucidated.•Oxygen transfer effects on intracellular reaction rates of B. subtilis were analysed.•Metabolic engineering sites and bottlenecks for rhGH production were determined.•Guides well designed genome reductions for B. subtilis host cell constructions.