RNase H-Assisted Imaging of Peroxynitrite in Living Cells with 5′-Boronic Acid Modified DNA

ACS Sensors(2016)

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摘要
RNase H is a ubiquitous intracellular enzyme able to selectively hydrolyze the RNA strand of an RNA/DNA hybrid duplex. In the present study, we demonstrate that 5′-boronic acid-modified oligonucleotides could inhibit RNase H activity; actvity which could subsequently be restored upon oxidation of the boronic acid moiety. This unprecedented dual property was exploited to engineer a new stimuli-responsive system for the chemoselective imaging of endogenous and exogenous peroxynitrite in RAW264.7 cells.
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关键词
RNase H,boronic acid,peroxynitrite,ADN,stimuli-responsive
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