Effects of jinggangmycin on peroxidase and esterase isozymes and phytotoxin from Rhizoctonia solani AG-1 IA, the causal agent of rice sheath blight. Pirinç kabuğu yanığı nedeni Rhizoctonia solani AG-1 IA tarafından üretilen peroksidaz, esteraz izozimleri ve fitotoksinler üzerinde jinggangmycin etkisi

Objective: To explore the effects of jinggangmycin on the pathogenicity of rice sheath blight. Methods: The effects of jinggangmycin on peroxidase and esterase isozymes from Rhizoctonia solani Kuhn AG-1 IA were compared using non-denaturing polyacrylamide gel electrophoresis (PAGE). Rs-toxins were obtained from the improved Richard medium amended with jinggangmycin at different concentrations. Subsequently, bioassays of Rs-toxin, including virulence to rice tissues, seedling wilting and inhibition to the seed germination were conducted. Results: The results of PAGE showed that when the jinggangmycin concentration increased to 50 mu g/mL, two of peroxidase isozyme bands became apparently weaker compared with the controls, indicating that jinggangmycin could considerably weaken the activity of the perox-idase isozyme. In addition, three extra bands of esterase isozyme were induced by jinggangmycin when the concentration increased to 50 mu g/mL, indicating that jinggangmycin could affect the metabolism of esterase isozyme. The bioassays of Rs-toxin showed that jinggangmycin could weaken the virulence of Rs-toxin in rice tissues. Conclusion: It was concluded that the control role of jinggangmycin in rice sheath blight might be due to the effects of jinggangmycin on the peroxidase and esterase isozymes and Rs-toxin virulence.