Synthesis and polymerase incorporation of β,γ-modified α-l-threofuranosyl thymine triphosphate mimics.

Three β,γ-modified α-l-threofuranosyl nucleoside triphosphates were synthesized. The β,γ-modified tTTPs undergo a single incorporation event with HIV RT but undergo multiple incorporations to form full-length product with engineered thermophilic polymerases.