Calibrated kallikrein generation in human plasma

D. Biltoft,J.J. Sidelmann,L.F. Olsen,Y. Palarasah,J. Gram

Clinical Biochemistry（2016）

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摘要

•New global method for measuring kallikrein generation potential in plasma•Fluorogenic substrate Boc-LKR-AMC was specific for kallikrein activity.•Signal was externally calibrated with α2M-kallikrein complexes.•Method detects abnormalities of the contact activation system.

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PK,FXI,HMWK,FXII,FXIIa,C1-inhibitor,α2M,CAT-method,NaN3,BSA,PBS,EDTA,FXII DEP,PK DEP,HAE,CTI,SBTI,MWCO,OSCAR,PEG-6000,Sephacryl column,Vivaspin sample concentrator,2HB plate,fluorometer

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