Secretory production of enzymatically active endo-β-1,4-mannanase from Bacillus subtilis by ABC exporter in Escherichia coli

Gyeong Tae Eom,Joon Young Oh,Ji Hyun Park,Jonggeon Jegal,Jae Kwang Song

Process Biochemistry（2016）

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摘要

•ABC exporter and secretion signals of P. fluorescens were reconstituted in E. coli.•The enzymatically active mannanase with optimized signal sequence was secreted by ABC exporter.•The uncleaved signal sequences did not alter the enzymatic properties of mannanase.

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关键词

Type I secretion system,Pseudomonas fluorescens ABC exporter,Bacillus subtilis endo-β-1,4-mannanase,Secretory production in Escherichia coli,C-terminal signal sequence

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