Deinococcus Fonticola Sp. Nov., Isolated From A Radioactive Thermal Spring In Hungary

A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium, designated strain FeSDHB5-19(T), was isolated from a biofilm sample collected from a radioactive thermal spring (Budapest, Hungary), after exposure to 5 kGy gamma radiation. A polyphasic approach was used to study the taxonomic properties of strain FeSDHB5-19(T), which had highest 16S rRNA gene sequence similarity to Deinococcus antarcticus G3-6-20(T) (96.5 %). The 16S rRNA gene sequence similarity to type strains of other Deinococcus species were 93.0 % or lower. The DNA G+C content of the draft genome sequence, consisting of 3.9 Mb, was 63.9 mol%. Strain FeSHDB5-19(T) was found to grow at temperatures of 10-32 degrees C (optimum, 28 degrees C) and pH 5-10 (pH 6.5-7.5) and tolerated up to 1.5 % NaCl (w/v) with optimum growth at 0-0.5 % NaCl. The predominant fatty acids (>10 %) were C-16:0 and C-16:1 c omega 7c. The cell-wall peptidoglycan type was A3 beta L-Orn-Gly(1-2). The whole-cell sugars were glucose and low amounts of galactose. Strain FeSDHB5-19(T) possessed MK-8 as the predominant respiratory quinone, typical of the genus Deinococcus. The polar lipid profile contained unidentified phosphoglycolipids and unidentified glycolipids. The isolate was found to be highly resistant to gamma (D 10 <8 kGy) and UV (D-10 similar to 800 J m(-2)) radiation. According to its genotypic, phenotypic and chemotaxonomic characteristics, strain FeSDHB5-19(T) represents a novel species in the genus Deinococcus, for which the name Deinococcus fonticola sp. nov. is proposed. The type strain is FeSDHB5-19(T) (=NCAIM B.02639(T) =DSM 106917(T)).