Expression of active barley seed ribosome-inactivating protein in transgenic wheat
Theoretical and Applied Genetics(2000)
摘要
Phenotypically normal, transgenic wheat ( Triticum aestivum , var. Frisal) plants expressing a barley seed ribosome-inactivating protein (RIP) were produced. Expression was controlled by an intron-enhanced cauliflower mosaic virus 35S promoter and has been completely stable over four generations so far, possibly due to matrix-associated regions (MARs) that flank the transgenes. An engineered fusion to a signal peptide derived from the barley seed ß-1,3-glucanase caused the transport of RIP to the apoplast. Activity of the accumulated protein could be shown by significant inhibition of a rabbit reticulocyte transcription/translation system. Plants expressing high levels of RIP were protected only moderately or not at all against infection by the fungal pathogen Erysiphe graminis .
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关键词
wheat transformation,ribosome-inactivating protein,anti-fungal protein,protein-targeting
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