The Expression Characteristics of Transcription Factors Regulated by Exogenous ABA in Winter Wheat ( Triticum aestivum ) under Cold Stress

‘Dongnong Dongmai1 (Dn1)’ is a cultivar of wheat ( Triticum aestivum L.) which may endure‒30°C in field with a reviving rate of 85%. ABA may enhance plant resistance. Aiming to reveal Dn1 coldresistant mechanism after exogenous ABA (exo-ABA) treated (control: non-ABA treated), some transcription factors (TFs) were selected and detected. Seedlings were prayed with exo-ABA at three-leaf stage, subsequently their tilling nodes were respectively sampled when it was at 5, 0,–10 and–25°C in field. Six TF genes ( TabZIP1 , TaWABI5 , TaMYB1 , TaMYB80 , TaNAC2 and TaWRKY80 ) were selected and their expression at different low temperature was detected, the results showed that: in non-ABA treated tilling nodes, the expression of six TF genes all reached their peak level at–10°C; in exo-ABA treated tilling nodes, the expression of five TF genes ( TabZIP1 , TaWABI5 , TaMYB80 , TaNAC2 and TaWRKY80 ) reached their peak level at–25°C, and particularly the expression level of TabZIP1 was the highest in five TFs. In order to further defined the biological function of TabZIP1 , the physical and chemical properties, protein secondary structure and the evolutionary tree was analyzed and predicted by some bioinformatics software or online program; TabZIP1 was localized in nuclear of tobacco ( Nicotiana tabacum ), which was observed by laser scanning confocal microscope (LSCM). This study initially exposed some TFs in Dn1 were upregulated to respond to the coldstress after exo-ABA treated, which indicated that a serious cold-responsive genes could be transcripted with the TFs assistance, and the results provided new theoretical basis to reveal the cold-reponsive mechanism of winter wheat.