Pluripotent stem cell-derived bile canaliculi-forming hepatocytes to study genetic liver diseases involving hepatocyte polarity.

•Functional cell polarity can be achieved in patient pluripotent stem cell-derived hepatocytes.•This will enable the study of autologous mutant proteins in biliary processes.•The Wilson disease-causing ATP7B-H1069Q mutation prevents its copper-induced polarized redistribution.•MEDNIK syndrome-causing AP1S1 mutations do not cause the expected defect in copper-stimulated redistribution of ATP7B.