Interleukin-27 promotes CD8+ T cell reconstitution following antibody-mediated lymphoablation.

Antibody-mediated lymphoablation is used in solid organ and stem cell transplantation and autoimmunity. Using murine anti-thymocyte globulin (mATG) in a mouse model of heart transplantation, we previously reported that the homeostatic recovery of CD8(+) T cells requires help from depletion-resistant memory CD4(+) T cells delivered through CD40-expressing B cells. This study investigated the mechanisms by which B cells mediate CD+ T cell proliferation in lymphopenic hosts. While CD8(+) T cell recovery required MHC class I expression in the host, the reconstitution occurred independently of MHC class I, MHC class II, or CD80/CD86 expression on B cells. mATG lymphoablation upregulated the B cell expression of several cytokine genes, including IL-15 and IL-27, in a 034-dependent manner. Neither treatment with anti-03122 mAb nor the use of IL-15R alpha(-/-) recipients altered CD8(+) T cell recovery after mATG treatment, indicating that IL-is may be dispensable for T cell proliferation in our model. Instead, IL-27 neutralization or the use of CD8(+) T cells inhibited CD8(+) T cell proliferation and altered the phenotype and cytokine profile of reconstituted CD8(+) T cells. Our findings uncover what we believe is a novel role of IL-27 in lymphopenia-induced CD8(+) T cell proliferation and suggest that targeting B cell-derived cytokines may increase the efficacy of lymphoablation and improve transplant outcomes.