Generation of Venus fluorochrome expressing transgenic handmade cloned buffalo embryos using Sleeping Beauty transposon.

Dharmendra Kumar,Papori Sharma,Kennady Vijayalakshmy,Naresh L Selokar,Pradeep Kumar,Rasika Rajendran,P S Yadav

Tissue and Cell（2018）

引用 9|浏览16

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摘要

•Integration of Venus constructs in buffalo fetal fibroblasts using Sleeping Beauty transposition.•Generation of transgenic handmade cloned buffalo embryos using Venus expressing donor cells.•Venus gene could be safely used as a marker of foreign genes in buffalo transgenesis.

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关键词

Buffalo,Developmental competence,SCNT,Transposon,Transgenesis

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