Correlation Of The Blood Test Results Obtained Between Assays Using Microliter-Scale Fingertip Blood Samples Collected With A Novel Blood Collection Device And Conventional Venous Blood Assays: A Secondary Publication In English

In this study, we investigated the correlations between biochemical and hematological test results obtained using microliter-scale fingertip blood samples collected with a newly developed blood collection device and those obtained using conventional venous blood. Eighty volunteer subjects were enrolled in this study. Blood samples were drawn from the fingertip of the ring finger by a single puncture, and 60-mu L samples were promptly and accurately aspirated into a blood collection chip. Then the chip was tightly sealed in a chip container and was shaken to mix the contents without dispersion. For biochemical tests other than that for HbAlc, blood was collected without anticoagulant and centrifuged to obtain 15 pi of serum which was then diluted with 190 mu L of physiological saline for the assay. For hematological tests and the test for HbAlc, the sample was assayed with blood collected using EDTA-2 K. Good correlations were obtained between the test results of the assay using fingertip blood and that using venous blood. The correlation coefficients were >= 0.97 for TG, T-CHO, HDL-C, LDL-C, GLU, ALT, gamma-GTP, UA, BUN, and HbAlc and >= 0.95 for WBC, RBC, Hgb, and Hct. These results suggest that our microliter-scale blood testing system is comparable to assays using venous blood and may be useful as a rapid and simple test to determine basic clinical parameters that are close to the reference intervals.