Skin suturing and cortical surface viral infusion improves imaging of neuronal ensemble activity with head-mounted miniature microscopes.

Xinjian Li,Vania Y Cao,Wenyu Zhang,Surjeet S Mastwal,Qing Liu,Stephani Otte,Kuan Hong Wang

Journal of Neuroscience Methods（2017）

引用 25|浏览8

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摘要

•Cortical surface virus infusion efficiently labels superficial layer neurons.•Scalp skin suturing after cranial window implants improves long-term optical clarity.•High yield neuronal ensemble imaging is achieved with head-mounted miniscopes.

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关键词

Cranial window,Scalp skin suture,Virus infusion,Cortical neuron ensemble,Miniaturized microscopy,Calcium imaging

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