CUTL1 induces epithelial-mesenchymal transition in non-small cell lung cancer.
ONCOLOGY REPORTS(2017)
摘要
The homeobox transcription factor CUTL1 has been associated with cellular proliferation and cell cycle progression, and CUTL1 functions as an oncogene. The aim of the present study was to investigate whether CUTL1 participates in epithelial-mesenchymal transition (EMT). The expression levels of CUTL1, E-cadherin, N-cadherin and Snail were determined by immunohistochemistry assay, immunofluorescence assay or real-time quantitative reverse transcription PCR. Their roles in non-small cell lung cancer (NSCLC) were assessed by functional analyses. Protein expression was detected by western blot analysis. The CUTL1 expression levels are higher in non-small cell lung cancer (NSCLC) tissues. High CUTL1 expression in NSCLC is associated with the mesenchymal-like phenotype. Mechanistically, CUTL1 upregulates transforming growth factor beta receptor I (T beta R-I) expression, and the T beta R-I inhibitor SB431542 abolishes EMT elicited by ectopic CUTL1 expression. Transforming growth factor beta (TGF-beta) signaling is essential for CUTL1-induced EMT in NSCLC cells. CUTL1 is downstream of TGF-beta signaling and CUTL1 is involved in the expression of the T beta R-I. This study indicates that CUTL1 may be a potential target for anti-lung cancer therapy.
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关键词
CUTL1,epithelial-mesenchymal transition,non-small cell lung cancer,transforming growth factor beta
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