Discovery of New Stilbene Antioxidants of the Bio-Elicited Peanut Sprout Powder (BPSP) and Longevity Extension of Mice Fed with BPSP-Supplemented Diets

Biosynthesis of peanut stilbenes, including resveratrol as the secondarymetabolites, could be enhanced by subjecting the kernels to germination andwound-stress. Investigations of the bio-elicited peanut sprout powder (BPSP)addressed on characterization of the comprising stilbenes and effectiveness in longevity extensiondeserves intensiveresearch. In this study, peanut kernels were subjected to germination andwound-stress in preparation of BPSP. The methanol extracts of BPSP were mediumpressure liquid chromatographic (MPLC) fractionated and semi-preparative HPLCrecovered and followedby instrumental identification and biological activity determinations of the isolatedstilbenes. In longevity experiments, 16 female 11-mon-old BALB/c mice and bothgenders of 12-mon-old ICR mice were daily fed with BPSP supplemented diets at doses of 0, 0.1 and 0.5 gBPSP/kg bw for 750 and 762 days, respectively. Based on chemical characterization, enrichedquantity of stilbenes in the BPSP up to ca. 1% (w/w) was detected. Two newstilbene compounds, namely, 4, 5’-dihydroxy-6’’-hydroxymethyl, 6’’-methylpyrano [2’’, 3’’: 3’, 4’] stilbene and 3, 4, 5’-trihydroxy-6’’,6’’-dimethylpyrano [2’’, 3’’: 3’, 4’]stilbene along with 5 known stilbenes were isolated. The 7 stilbenes exhibitedpotent antioxidative andantiglycative activities andvaried with structure-activity nature. Based on the resultant survival curves and averagelifespans of both mouse models, basal diets supplemented with BPSP areeffective to extend mouse longevity by a dose dependent manner. It is of merit todemonstrate that peanut kernels as a potent producer could be bio-elicited tobiosynthesize a broad spectrum of bioactive stilbenes to prepare BPSP which iseffective to extend mouse longevity as science-evidenced by the two long-termanimal experiments.