Rapid identification of causal mutations in tomato EMS populations via mapping-by-sequencing

Virginie Garcia,Cécile Bres,Daniel Just,Lucie Fernandez,Fabienne Wong Jun Tai,Jean-Philippe Mauxion,Marie-Christine Le Paslier,Aurélie Bérard,Dominique Brunel,Koh Aoki,Saleh Alseekh,Alisdair R Fernie,Paul D Fraser,Christophe Rothan

NATURE PROTOCOLS（2016）

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摘要

Garcia et al. describe a protocol for mapping-by-sequencing in Tomato. After selecting EMS mutants displaying a phenotype of interest, mutants are back-crossed and causal mutations are identified in the F 2 population using sequencing-based approaches.

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关键词

Next-generation sequencing,Genetic mapping,Plant breeding,Plant genetics

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