Routine in vitro culture of P. falciparum gametocytes to evaluate novel transmission-blocking interventions

Michael J Delves,Ursula Straschil,Andrea Ruecker,Celia Miguel-Blanco,Sara Marques,Alexandre C Dufour,Jake Baum,Robert E Sinden

NATURE PROTOCOLS（2016）

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摘要

This is a relatively simple protocol for the routine culture of P. falciparum gametocytes in vitro . The functional viability of gametocytes is assessed, and they can then be used to evaluate transmission-blocking activity of drugs.

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关键词

Parasite biology,High-throughput screening,Isolation,separation and purification,Malaria

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