High-quality full-length immunoglobulin profiling with unique molecular barcoding

M A Turchaninova,A Davydov,O V Britanova,M Shugay,V Bikos,E S Egorov, V I Kirgizova,E M Merzlyak,D B Staroverov,D A Bolotin,I Z Mamedov,M Izraelson,M D Logacheva, O Kladova,K Plevova,S Pospisilova,D M Chudakov

NATURE PROTOCOLS（2016）

引用 143|浏览45

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摘要

This protocol enables high-throughput sequencing analysis of the full-length immunoglobulin repertoires in human and mouse using unique molecular identifiers (UMIs) and asymmetric paired-end sequencing.

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关键词

Bioinformatics,Next-generation sequencing,Adaptive immunity,Immunological techniques

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