6-Methylsulfinylhexyl isothiocyanate prevents high-fat diet-induced fatty liver but fails to attenuate hepatic iron accumulation in mice.

6-Methylsulfinylhexyl isothiocyanate (6-MSITC) is a bioactive ingredient present in Japanese horseradish (wasabi) and has been shown to be an Nrf2 activator. To determine whether 6-MSITC ameliorates hepatic steatosis and iron accumulation, wild-type and Nrf2-null mice were fed the following diets for 12 weeks: 1) control diet, 2) high-fat diet (HFD), 3) HFD plus 6-MSITC (10mg/Kg/day ip), 4) HFD for 6 weeks followed by an iron-supplemented HFD for 6 weeks (HFD/Iron), 5) HFD/Iron plus 6-MSITC. The HFD increased hepatic triglycerides in both genotypes and 6-MSITC suppressed increased hepatic triglycerides in wild-type mice but did not reduce these triglycerides in Nrf2-null mice. Compared to the HFD and HFD/Iron groups, respectively, 6-MSITC did not block hepatic iron accumulation in either genotype.This article is protected by copyright. All rights reserved.