Immunofluorescence staining protocol for STED nanoscopy of Plasmodium-infected red blood cells.

Ann-Kathrin Mehnert,Caroline Sophie Simon,Julien Guizetti

Molecular and Biochemical Parasitology（2019）

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摘要

•Omitting glutaraldehyde from fixative improves immunofluorescence staining.•STED nanoscopy is readily applicable to infected red blood cells.•Intranuclear microtubules are nucleated from distinct sites.

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关键词

Malaria,Plasmodium falciparum,Blood stage,Immunofluorescence,STED,Super-resolution

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