Tessaracoccus Flavus Sp Nov., Isolated From The Drainage System Of A Lindane-Producing Factory

Strain RP1(T), a Gram-stain-positive, non-motile, non-spore-forming, coccus-shaped bacterium, was isolated from drainage of India Pesticides Limited, a lindane-producing unit situated at Chinhat, Lucknow, India. 16S rRNA gene sequence analysis revealed that strain RP1(T) belongs to the family Propionibacteriaceae and was closely related to the members of the genus Tessaracoccus with a similarity range of 95.4-97.6 %. Strain RP1(T) was facultatively anaerobic, oxidase-negative, catalase-positive and capable of nitrate reduction. Strain RP1(T) contained peptidoglycan type A3 gamma', with LL-diaminopimelic acid as the diagnostic diamino acid and glycine at position 1 of the peptide subunit. The major cellular fatty acid of strain RP1(T) was anteiso-C-15 (: 0) but a significant amount of iso-C-14 : 0 was also detected. MK-9(H-4) was the major respiratory quinone and polyamines detected were spermine and spermidine. The polar lipids included diphosphatidylglycerol, phosphatidylglycerol, two unknown glycolipids and two unknown phospholipids. The G+ C content of the DNA was 66.7 mol%. The levels of DNA-DNA relatedness between RP1(T) and Tessaracoccus lubricantis KSS-17Se(T), Tessaracoccus oleiagri SL014B-20A1(T) and Tessaracoccus flavescens SST-39(T) were 49.8, 34.8 and 23.5 %, respectively. Based on the phenotypic and phylogenetic data presented, strain RP1(T) can be differentiated from previously described species of the genus Tessaracoccus, and thus represents a novel species, for which the name Tessaracoccus flavus sp. nov. is proposed. The type strain is RP1(T) (=DSM 100159(T) =MCC 2769(T) =KCTC 39686(T)).