Bio-guided identification of proteins for the diagnosis of cysticercosis in swine.

Priscilla Nativel,Anjanirina Rahantamalala, Sitraka Ramiandrisoa, Virginie Rasoamampianinaa,Magalie Duchateau,Julia Chamot-Rooke,Remy Guebey,Harentsoaniaina Rasamoelina-Andriamanivo,Ronan Jambou

Veterinary Parasitology（2016）

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摘要

•Diagnostic of cysticercosis must be drawn to gate set for animals, to sustain control strategy.•Bioguided identification of proteins from the liquid of the cyst was done using serums from positive and negative animals.•Exchange chromatography and 2D-electrophoresis was used to identify protein candidates to develop a diagnostic test.•Mass spectroscopy allowed to identify 18 new proteins and 9 were selected for further development

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关键词

Madagascar,Taenia solium,Cysticercosis,2D electrophoresis,LC/MS-MS

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