Molecular crowding facilitates assembly of spidroin-like proteins through phase separation

Laura Lemetti,Sami-Pekka Hirvonen,Dmitrii Fedorov,Piotr Batys,Maria Sammalkorpi,Heikki Tenhu,Markus B. Linder,A. Sesilja Aranko

European Polymer Journal（2019）

引用 21|浏览24

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摘要

•The effect of a crowding agent on the self-coacervation of a silk-like protein was characterized.•The thermodynamics of coacervation was studied.•Driving forces and mechanisms of phase separation are discussed.

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关键词

Liquid-liquid phase separation,Coacervation,Self-coacervation,Molecular crowding,Fusion protein,Isothermal titration calorimetry,FRAP,Biopolymer,Spidroin

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