Sugar and ice: Immunoelectron microscopy using cryosections according to the Tokuyasu method

•Versatile method for preparation of cryosections for immunolabeling of fixed samples.•Target molecules are accessible to antibodies from the section surface.•Antigenicity is preserved by mild fixation and by keeping an aqueous environment.•Correlative light and electron microscopy workflows are established.•The use of particulate markers allows quantification and statistical evaluation.