Controlled Chemical Modification Of Biomolecules By Femtosecond Laser In Polar Liquids
2018 INTERNATIONAL CONFERENCE LASER OPTICS (ICLO 2018)(2018)
摘要
Covalent chemical modification of peptides and human insulin by ultrashort laser pulses in room-temperature polar liquids show highly nonlinear scaling with laser intensity. Mass spectrometry analysis of the products obtained in acetonitrile-water and methanol-water, using isotope-marked liquids, and argon sparging demonstrates the participation of dissolved oxygen and interaction of the biomolecules with the laser-modified molecules derived from the liquids.
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关键词
molecular biophysics, photochemistry, protein engineering, biochemistry, peptide, proteins
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