Abstract 3239: Molecular biomarkers and indoor household air pollution in a rural area of China

To explore the influence of fuel source used for home heating and cooking on biomarkers of potential genomic instability, we analyzed biological samples obtained from a cross-sectional study of 104 female never-smokers enrolled from 30 villages in Xuanwei and Fuyuan in Yunnan Province, a rural region with a high incidence of lung cancer in China. Study subjects who used primarily smoky (i.e., bituminous) coal (n=80), smokeless coal (i.e., anthracite) (n=14) and wood (n=10) were analyzed. Personal 24-h air PM2.5 and polycyclic aromatic hydrocarbon concentrations were measured over two consecutive days in each household. DNA was extracted from peripheral white blood cells. We assayed molecular biomarkers of potential genomic instability including the Alu copy number to albumin gene (ALB) ratio (Alu/ALB), 5.8S ribosomal DNA to ALB ratio (5.8S rDNA/ALB) and 18S ribosomal DNA to ALB ratio (18S …