Molecular Cloning of AZIN2 and its Expression Profiling in Goose Tissues and Follicles

Ornithine decarboxylase antizyme inhibitor 2 (AZIN2) plays key roles in regulating the biosynthesis and content of polyamines and other amines such as serotonin and histamine. The AZIN2 coding sequence in the goose was cloned and analysed in this experiment. AZIN2 expression levels in goose tissues and follicles were also measured. A full-length AZIN2 coding sequence (GenBank accession no. MF939648) encoding a 457-amino acid protein was 1374 bp in length. The molecular weight of putative AZIN2 protein was 49.10 kDa. The mRNA expression of AZIN2 was not observed in heart, adrenal gland, breast muscle, thigh muscle and pineal gland tissues in the goose. The highest and lowest expression levels of AZIN2 in all examined tissues were observed in cerebrum and kidney tissues, respectively. The mRNA expression levels of AZIN2 in the cerebrum, cerebellum and hypothalamus were 61.95-, 15.87- and 15.04-fold higher compared to the liver, respectively. The mRNA expression of AZIN2 was observed in all examined follicles. The mRNA expression level of AZIN2 was 6.48-fold higher in the Fl follicle compared to the SWF. Taken together, AZIN2 mRNA has a restricted pattern of expression in the goose. AZIN2 may play important roles in modulating physiological functions of the brain and regulating follicular development and ovulation of goose ovaries.