Wide-field in situ multiplexed Raman imaging with superresolution

Because of the fingerprint-like specificity of its characteristic spectrogram, Raman spectral imaging has been applied widely in various research areas. Using a combination of structured illumination with the surface-enhanced Raman scattering (SERS) technique, wide-field Raman imaging is developed with a significant improvement in spatial resolution. As a result of the relatively narrow Raman characteristic peaks, optically encoded SERS nanoparticles can be used to perform multiplexed imaging. The results show excellent superresolution wide-field multiplexed imaging performance. The developed technique has extraordinary potential for applications in biological imaging and other related fields. (c) 2018 Chinese Laser Press