Microbial expression systems for membrane proteins

Marvin V. Dilworth,Mathilde S. Piel,Kim E. Bettaney,Pikyee Ma,Ji Luo,David Sharples,David R. Poyner,Stephane R. Gross,Karine Moncoq,Peter J. F. Henderson,Bruno Miroux,Roslyn M. Bill

Methods（2018）

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摘要

•Microbes have generated 71% of recombinant membrane proteins with unique structures.•Data on usage of strains, tags and promoters are discussed.•Detergents used for solubilization and crystallization are presented.

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关键词

Recombinant membrane proteins,Expression plasmid vector,Tag,Promoter,Detergent

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