Assessing Field Prunus Genotypes for Drought Responsive Potential by Carbon Isotope Discrimination and Promoter Analysis
AGRONOMY-BASEL(2018)
摘要
In order to improve the effectiveness of breeding practices for Prunus rootstocks, it is essential to obtain new resistance resources, especially with regard to drought. In this study, a collection of field-grown Prunus genotypes, both wild-relative species and cultivated hybrid rootstocks, were subjected to leaf ash and carbon isotope discrimination (Delta C-13) analyses, which are strongly correlated to water use efficiency (WUE). Almond and peach wild relative species showed the lowest Delta C-13 ratios, and therefore, the highest WUE in comparison with hybrid genotypes. In addition, drought-related cis-regulatory elements (CREs) were identified in the promoter regions of the effector gene PpDhn2, and the transcription factor gene DREB2B, two genes involved in drought-response signaling pathways. The phylogenetic analysis of these regions revealed variability in the promoter region sequences of both genes. This finding provides evidence of genetic diversity between the peach- and almond-relative individuals. The results presented here can be used to select Prunus genotypes with the best drought resistance potential for breeding.
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关键词
almond wild-relative species,ash content,DREB2B transcription factor,peach wild-relative species,PpDhn2,rootstock,water use efficiency
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