PD33-02 MESENCHYMAL STEM CELLS IMPROVED BLADDER FUNCTION AND HISTOPATHOLOGICAL FEATURES IN PROTAMINE SULFATE-LIPOPOLYSACCHARIDE INDUCED INTERSTITIAL CYSTITISIN RAT BLADDER

You have accessJournal of UrologyStem Cell Research: Stem Cell Research I1 Apr 2018PD33-02 MESENCHYMAL STEM CELLS IMPROVED BLADDER FUNCTION AND HISTOPATHOLOGICAL FEATURES IN PROTAMINE SULFATE-LIPOPOLYSACCHARIDE INDUCED INTERSTITIAL CYSTITISIN RAT BLADDER Chae-Min Ryu, Hwan Yeul Yu, Ju Young Han, Daeheon Choi, Jung-Hyun Shin, Dong-Myung Shin, and Myung-Soo Choo Chae-Min RyuChae-Min Ryu More articles by this author , Hwan Yeul YuHwan Yeul Yu More articles by this author , Ju Young HanJu Young Han More articles by this author , Daeheon ChoiDaeheon Choi More articles by this author , Jung-Hyun ShinJung-Hyun Shin More articles by this author , Dong-Myung ShinDong-Myung Shin More articles by this author , and Myung-Soo ChooMyung-Soo Choo More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2018.02.1557AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES To evaluate the therapeutic effect of mesenchymal stem cells (MSCs)inthe chronic interstitial cystitis (IC) rat model. METHODS To make IC rat model female 8-week-old Sprague-Dawley ratswere given protamine sulfate (PS, 10mg) and lipopolysaccharide (LPS, 750ug) weekly for 5 weeks. One week after final administration of PS/LPS, rat were divided into five groups: sham (n=10); PBS (n=10, IC); 0.25 million of MSCs (n=10, IC+MSCs); 0.5 million of MSCs (n=10, IC+MSCs); 1 million of MSCs (n=10, IC+MSCs) were injected into the submucosal layer of the anterior wall and dome of the bladder.The therapeutic effect of MSCs was examined by awakecystometry,histological and gene expression analysis 1 week after MSCs injection. RESULTS IC rat groupexhibited irregular voiding frequency, decreased inter-contraction intervals, micturition volume and increased residual volume. A single injection of MSCs significantly improved most of voiding parameters by increased the inter-contraction interval, increased micturition volume and decreased residual volume. The bladder of IC group rats were characterized with severe denudated urothelium with inflammation, increased mastcell infiltration and paralleled with down-regulation of Wnt-8a, Wnt-8b and Wnt-11. MSCs therapy significantly upregulatedShh and Wnt family genes (Smo, Wnt5a, Wnt8a, Wnt8b, Wnt10a, and Wnt11) as well as their downstream growth factors (Igf1, Igf2) which were characteristically downregulated in bladders of the LPS-IC group. A single transplantation of 1X106 MSCs (LPS-IC + MSCs group) significantly ameliorated thesedefective voiding parameters.Of note, a single injection of MSCs significantly not only improved the bladder voiding parameters but also reversed the histological and gene expression alternations characteristic for IC bladder. CONCLUSIONS We demonstrate that MSC therapy have therapeutic effect to repair voiding function and regenerate denudated urothelium and stabilize mast cell infiltration in the most proper IC rat model. © 2018FiguresReferencesRelatedDetails Volume 199Issue 4SApril 2018Page: e651 Advertisement Copyright & Permissions© 2018MetricsAuthor Information Chae-Min Ryu More articles by this author Hwan Yeul Yu More articles by this author Ju Young Han More articles by this author Daeheon Choi More articles by this author Jung-Hyun Shin More articles by this author Dong-Myung Shin More articles by this author Myung-Soo Choo More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...