Visualization of Chromatin Loci with Transiently Expressed CRISPR/Cas9 in Plants
CYTOLOGIA(2017)
摘要
Labeling of specific DNA sequences in living organisms is important for understanding the mechanism of gene regulation during development, differentiation, and environmental adaptation. Here we describe a CRISPR/Cas9-based chromatin visualization system in plants. We adapted the mammalian nuclease-dead Cas9-fluorescent protein (dCas9-FP) system to a plant expression vector. Transient expression of dCas9-FP and single guide RNA (sgRNA) for telomeric sequences efficiently labeled telomere repeats in tobacco.
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关键词
CRISPR-dCas9,Chromatin dynamics,Live cell imaging,Nicotiana tabacum
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