SnoRNA33 as a New Therapeutic Target in Ischemia/ Reperfusion Induced Myocardial Injury

Introduction: SnoRNA33 is a H/ACA RNA, which is predicted to guide the pseudouridylation of 28S rRNA U4966. Other than this information, roles and mechanisms of snoRA33 in human physiology and pathology are largely unknown. Our preliminary data showed that snoRNA33 expression was highly upregulated in mouse myocardial ischemic/reperfusion (I/R) injury. This study is intended to evaluate the utility of snoRNA33 as a new preventive and therapeutic target in I/R induced mouse myocardial injury and to investigate underlying cell and molecular mechanisms. Hypothesis: We hypothesize that snoRNA33 may be a genetic risk factor and therapeutic target in CAD. Methods: Male C57BL/6J mice, 8-12week-old, were subjected to either sham operation or myocardial I/R by a 40 min occlusion of the left anterior descending coronary artery followed by 3 h reperfusion without or with i.v. morpholino-snoRNA33 antisense oligo (MAO), or its scrambled control (MAOsc) either prior to or during the operation. 24 h later, mice were sac...