Ammonolysis of (5 S )- N -( tert -butoxycarbonyl)-5-(methoxycarbonyl)-2-pyrroline with immobilized Candida antarctica lipase B (CAL B) in a packed bed reactor

Harini Tirunagari,Lavanya Kuna,Basetty Shalini,Kumaraguru Thenkrishnan

Process Biochemistry（2018）

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摘要

•Ammonolysis of ester with a high conversion was carried out in a packed-bed reactor.•Immobilized Candida antarctica lipase B (CAL B) used as a catalyst.•Reaction follows Michaelis-Menten kinetics with competitive product inhibition.•Continuous production without chromatographic separation.

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关键词

Biocatalysis,Immobilised enzymes,Fixed-bed bioreactors,Lipase,Kinetic parameters,Ammonolysis,Saxagliptin

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