High-Throughput Single-Cell Dna Sequencing Of Aml Tumors With Droplet Microfluidics

To enable the characterization of genetic heterogeneity in tumor cell populations, we developed a novel microfluidic approach that barcodes amplified genomic DNA from thousands of individual cancer cells confined to droplets. The barcodes are then used to reassemble the genetic profiles of cells from next generation sequencing data. Targeted sequencing of genes frequently mutated in AML uncovered complex clonal architecture within AML tumors that was not observable with bulk sequencing.