A novel acoustic cell processing platform for cell concentration and washing

FloDesign Sonics has developed a technology to enable a single use (gamma irradiated) continuous cell concentration and wash application for manufacturing of cell-based therapies. The device has been designed to be able to process several liters of a suspended cell culture, e.g., T-cells, at concentrations of 1 to 10 M cells/ml. The cell suspension flows through the device and the acoustic radiation force field is used to trap and hold the cells in the acoustic field. After concentrating the cells, one or multiple washing steps are accomplished by flowing the washing fluid through the device, using the acoustic field to trap the cells while displacing the original cell culture fluid. The holdup volume of the device is about 30 ml. Results are shown for prototypes with a 1x0.75 inch flow chamber driven by 2 MHz PZT-8 transducers operating at flow rates of 1-2L/h, measured cell recoveries of 90% have been achieved with concentration factors of 20 to 50 for Jurkat T-cell suspensions, depending on cell concen...