Alterations Of Molecular Pattern In Clinical Biospecimen By Pre-Analytical Conditions.

e22263 Background: Biomarker analysis and patient stratification in the clinical situation is highly affected by the quality of biospecimen, which strongly depends on the pre-analytical conditions in which they were acquired. Warm and cold ischemia time that tissues are exposed to is of critical importance. Insufficient quality of such tissues may lead to spurious results and data misinterpretation resulting in biased stratification of patients. The present study was conducted to gain a better understanding of the molecular effects of warm and cold ischemia and consequences for clinical decision making. Methods: Normal and colorectal cancer (CRC) tissues from patients (n = 50) were analyzed by comparing endoscopy samples with samples fixed within 10, 20 and 45 minutes post surgery (cold ischemia). In addition, normal liver tissue (n = 43) before and after 10 minutes of warm ischemia were collected and compared to normal and metastatic liver tissue samples. Gene expression was analyzed using Affymetrix Gen...