Simultaneous Detection Of Multiple Key Genes In Chinese Non-Small Cell Lung Cancer Was Feasible In Clinical Practice.

e19149 Background: The identification of genetic mutational profiles is significant in personalized management of non-small cell lung cancer (NSCLC). Previous techniques are limited to analyzing only a single or a few mutations together. The study was to investigate the feasibility of simultaneously rapid detection of several key genes in a Chinese cohort. Methods: A multiplexed PCR-based assay (SnapShot) and GeneScan were integrated to genotype hotspots across over 9 key genes with pertinence to current or potential targeted therapy in NSCLC. Clinicalpathologic features, distribution frequency of individual gene and mutation overlap were reviewed. Results: Totally 45 treatment-naive and 44 treated NSCLC patients with eligible tumor specimens were enrolled into the study. Eighty-four percent of the cohort was with adenocarcinoma, and 54% of patients were smokers. EGFR, KRAS, NRAS, PTEN, BRAF, PIK3CA, MEK1, HER2 and BIM were simultaneously analyzed in a platform. Forty-three percent of patients were classi...